Methylcobalamin in Chlorella vulgaris:
Kumudha et al hat geschrieben:Abstract
Vitamin B12 is among the most essential biomolecules required for crucial metabolic processes in humans. Vitamin B12 was extracted from Chlorella vulgaris biomass under aqueous conditions, partially purified by passing the extract through amberlite XAD-2, Sep-Pak columns, and further purified by HPLC. The target peak eluent was subjected to characterisation by tandem mass spectrometry (MS/MS), selected ion recording (SIR) and multiple reaction monitoring (MRM) and identified as methylcobalamin (Me-Cbl). Quantification of Me-Cbl was carried out by microbiological and chemiluminescence methods, and found to be 29.87±2 μg/100 g and 26.84±2 μg/100 g dry weight, respectively. The presence of Me-Cbl was further substantiated using gold nanoparticle (AuNPs) based aptamer analysis, and found to be 28.02±2 μg/100 g dry weight. Good similarity was observed among all the methods. Methylcobalamin, a form of vitamin B12 was identified in C. vulgaris and this finding enhances its use as a nutritional supplement.
https://www.ncbi.nlm.nih.gov/pubmed/25306351
Chlorella pyrenoidosa als Supplement:
Merchant et al hat geschrieben:Abstract
Since vitamin B12 occurs in substantial amounts only in foods derived from animals, vegetarians and particularly vegans are at risk of developing deficiencies of this essential vitamin. The chlorella used for this study is a commercially available whole-food supplement, which is believed to contain the physiologically active form of the vitamin. This exploratory open-label study was performed to determine if adding 9 g of Chlorella pyrenoidosa daily could help mitigate a vitamin B12 deficiency in vegetarians and vegans. Seventeen vegan or vegetarian adults (26-57 years of age) with a known vitamin B12 deficiency, as evidenced by a baseline serum methylmalonic acid (MMA) level above 270 nmol/L at screening, but who otherwise appeared healthy were enrolled in the study. Each participant added 9 g of C. pyrenoidosa to their daily diet for 60 ± 5 days and their serum MMA, vitamin B12, homocysteine (Hcy) levels as well as mean corpuscular volume (MCV), hemoglobin (Hgb), and hematocrit (Hct) were measured at 30 and 60 days from baseline. After 30 and 60 days, the serum MMA level fell significantly (P < .05) by an average ∼34%. Fifteen of the 17 (88%) subjects showed at least a 10% drop in MMA. At the same time, Hcy trended downward and serum vitamin B12 trended upward, while MCV, Hgb, and Hct appeared unchanged. The results of this work suggest that the vitamin B12 in chlorella is bioavailable and such dietary supplementation is a natural way for vegetarians and vegans to get the vitamin B12 they need.
https://www.ncbi.nlm.nih.gov/pubmed/26485478
Jack Norris über diese Studie:
http://jacknorrisrd.com/chlorella-shown ... warranted/
Kommentar von Christian Koeder zum Artikel von Jack Norris:
Christian Koeder hat geschrieben:Yes, the 45 chlorella tablets per day seems unrealistic and expensive. But maybe this will make some vegans happy, so we can say “we could take chlorella, but cyanocobalamin is cheaper”
Also the chlorella “treatment” seemed to work for some, but not for others –>
15 of 17 participants had MMA >271 nmol/L at baseline –> 9 (participants #1, 4, 6, 8, 12, 13, 14, 16, 17) out of these 15 could lower their MMA below 271 nmol/L
– Note that MMA for participant #17 was lower at 30 days than at 60 days, i.e. inspite of increased intake of chlorella, MMA went up again but it stayed within the normal range.
– Note that in all of these above participants with successful “MMA-response” (participants #1, 4, 6, 8, 12, 13, 14, 16, 17) the vitamin B12 serum concentration also increased.
– Note that in 4 out of these 9 participants with good “MMA-response” homocysteine concentration was also corrected (went from above to below 10 µmol/L).
7 of 17 participants had Hcy >10 µmol/L at baseline –> 4 (participants #1, 8, 14, 17) out of these 7 could lower their Hcy below 10 µmol/L
That means only 15 of the 17 participants had increased MMA at the beggining, and out of those 9 could lower their MMA and 4 of could lower both MMA and Hcy by taking the 45 chlorella tablets.
Pflanzliche B12 Quellen - Artikel von Jack Norris:
https://veganhealth.org/vitamin-b12-plant-foods/
Jack Norris hat geschrieben:Conclusion About Organic Produce as a B12 Source for Vegans
The suggestion that humans have ever relied on uncleaned, organic produce for vitamin B12 doesn’t have any reliable evidence at this time.
Given the number of vegans who’ve suffered from B12 deficiency, and that vegans have traditionally interest in organic foods in the U.S. and U.K., it’s safe to assume that organic foods normally don’t provide B12. Only until organic foods are chosen randomly from markets and grocery stores throughout the country (or world) and are consistently shown to decrease MMA levels will someone not be taking a considerable risk in relying on organic foods for B12.
Finally, since the vegan movement’s aim is to eliminate cows on farms, relying on organic foods for vitamin B12 isn’t a long-term solution for providing vitamin B12 for vegans.
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